Figure 2 of
Yuan, Mol Vis 2004;
10:351-360.Figure 2 of
Yuan, Mol Vis 2004;
10:351-360.
Figure 2. Nucleotide sequence and the PCR amplification of the βIGH3 region
A: Putative transcription factor-binding sites within 1 Kb of the 5'-flanking region of the βIGH3 gene, as determined by the Proscan, TFSEARCH, and MatInspector databases. The term SBE indicates putative Smad binding elements. The start codon (ATG) is the last three bases. The asterisk represents the transcription starting site at -65 bp position. B: The proximal regions of mouse and human βIGH3 promoter are highly conserved. Numbering is relative to the ATG start codon. C: Effects of DMSO on the synthesis of 1 Kb fragments. PCR products were analyzed on a 0.8% agarose gel. Lane 1 shows the PCR reaction without DMSO, showing no apparent band. Lane 2 shows the PCR reaction with 1% DMSO, showing a weak 1 Kb cDNA band. Lane 3 shows the PCR reaction with 5% DMSO, showing a strong 1 Kb cDNA band.
-991 -981 -971 -961 -951
CTTCATGGAA CATCATTGGC TTGGGCTACG GATCTTCCCA AATTGGAGCT
IK2
-941 -931 -921 -911 -901 -891
TTTTCACAAA GGGCTTAGGT CTCACTCATT CTATTAATCC ATCTGTGTCT CCCCAGGGCT
SBE-1 SBE-2
-881 -871 -861 -851 -841 -831
AGCAGTGCCA AGTAACTGAC AGGTGATTAA TAGATGCTTG GGTAAGTATC ACCTCTTTAC
MYOD
-821 -811 -801 -791 -781 -771
CATGTGACAA TTTGTTTACC TGCCTTGAGC TCCTCCAGGG CAGGACTCTT GCCTTTGCAG
USF CEBPB -
-761 -751 -741 -731 -721 -711
AATCTATCTG GCAGGTACTG TTGCAGAGAT GTTTACTGAA GAAGGGAATG AATTAGTACC
-GATA1--------
-701 -691 -681 -671 -661 -651
AAGGTGAGGA CCCCACCCTT CCCCACGGGC TCCAAAAGCA GCTTAGAGCC CAACAAAACC
MZF1 Sp1
-641 -631 -621 -611 -601 -591
TGCCCCACAT TTTTGGCGTT TCTGTGGATC ACACGATTTA CTCATCTGTC TTTCAATGAG
E2F SBE-3
-581 -571 -561 -551 -541 -531
CATGACAGGT GGGGTGGGGG TGGAGGGATT AGAGATTGAG GAGCTGGGGA GGGTGGTCAG
MYOD
-521 -511 -501 -491 -481 -471
CTCCTGGGGT GCAGAAACAA GTCTGATGGG CCATGGTGTT CTGGGAATCA GCACTGCCTC
SBE-4 --STAT---- IK2
-461 -451 -441 -431 -421 -411
CCCTCACCCC TCCCTGCAGT GTTTTGTAGC CTCAAGATCA GTGAGGGAAT CTTCGGGCCC
MZF1 HSF1
-401 -391 -381 -371 -361 -351
CCAGCATGCA GGACCGAAGC CCCCGAGACA GCTGTCCCTC AGTCCCAAGG TCCCCATTTG
SBE-5 MZF1
-341 -331 -321 -311 -301 -291
GAAGCAGCCA CAGGAGGCCT AAGGGACCTA TACCCTTGGT TTGAGGAAGA CTGTGGCGAG
-281 -271 -261 -251 -241 -231
GGAGAGAGGG AGGGAGGGCT GGCAGTGAGG GCAAGGGCTG GGAAAACTGA GCACGGGCAC
IK2
-221 -211 -201 -191 -181 -171
AGTGCGGGAG CGGGTGGGTG CCCAGGGCAG CCAGGGGCGC ACGGGTTGGG AGGCGCCAGG
-161 -151 -141 -131 -121 -111
CGGCCCGCCC TCCTTGCACG GGCCGGCCCA GCTTCCCCGC CCCTGGCGTC CGCTCCCTCC
Sp1 ---MZF1- Sp1
-101 -91 -81 -71 -61 -51
CGCATCGCGC TTACTTAACC TGGCCCGGGC GGCGGAGGCG CTCTCACTTC CCTGGAGCCG
CEBPB ---VBP----- Sp1 *
-41 -31 -21 -11 -1
CCCGCTTGCC CGTCGGTCGC TAGCTCGCTC GGTGCGCGTC GTCCCGCTCC ATG
|
2B
-110 -51
Human CGCTCGCAGCTTACTTAACCTGGCCCGGGCGGCGGAGGCGCTCTCACTTCCCTGGAGCCG
Mouse --CTCGG-GC-TACTAAACCCAGCGCGCGCTGCCCCGCTGCTCTCACTTCACCAGCACCG
-50 -1
Human CCCGCTTGCCCGTCGGTCGCTAGCTCGCTCGGTGCGCGTCGTCCCGCTCC ATG
Mouse CCCGC--GCCTG-CTTTCA-TCG-TGGGTCCG--CGCGTGCTCCAGCTCC ATG
|
2C
