Table 1 of
Ottino, Mol Vis 2004;
10:341-350.Table 1 of
Ottino, Mol Vis 2004;
10:341-350.
Table 1. Primer sequences and amplification conditions
The sequences listed in the table correspond to primers taken from the cited references.
Optimal
Annealing cycle Product
Gene Primer sequence temp (°C) number size
-------------------------- -------------------------------- --------- ------- -------
Collagenase (MMP-1) [26] tcagttcgtcctcactccag 55 32 322
ttggtccacctgtcatcttc
Gelatinase A (MMP-2) [26] cccccaaaacggacaaagag 60 35 315
cacgagcaaaggcatcatcc
Gelatinase B (MMP-9) [26] aaactggatgacgatgtctgcgtcccg 58 35 362
acctgttccgctatggttacacccgcgta
TIMP-2 [26] gtagtgatcagggccaaag 60 35 416
ttctctgtgacccagtccat
MT1-MMP [46] gcccattggccagttctggcggg 55 35 530
cctcgtccacctcaatgatgatc
VEGF165 [27] cttgctgctgtacctccaccat 60 35 341
tgttgtgctgtaggaagctcatct
VEGF A (all isoforms) [47] gcggaattcatcatgcggatcaaacctcacca 65 30 470
tacggatcctccggacccaaagtgctc
FLT-1 [48] cccacttgccatcataagg 60 35 483
caccatacctcctgcgaaacct
KDR [49] atagatggtgtaacccggagtgac 62 35 360
tgggattggtaaggatgacagtgt
NP-1 Receptor [50] caacgataaatgtggcgatact 62 35 820
tatactgggaagaagctgtgat
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