Figure 2. Hypoxia induces MMP-2 but not MMP-9 secretion from choroid-retinal EC

The zymogram represents MMP-2 levels detected in conditioned medium (10 μl) collected from EC exposed to 2 or 4 h of hypoxia followed by reoxygenation for 12 h. The position of the active MMP-2 enzyme (68 kDa), visible as a clear band, was confirmed by comparison to APMA-activated purified human MMP-2. The position of the APMA-activated human granulocyte MMP-9 standard is visible as an 82 kDa band. Lane C is normoxic control cultures. The experiment was repeated twice with similar results.