Table 1 of
Koizumi, Mol Vis 2004;
10:328-340.
Table 1. RT-PCR primers
The outer primers were used for reverse transcription and the first-round PCR. A common outer primer pair was used to amplify mRNAs for HCN2-4. The inner primers were used for the nested second-round PCR. HCN2-4 were amplified using gene specific forward primers and a common reverse primer.
Size Primer name Primer sequence (bp) ------------ ---------------------------------- ---- HCN1 outer Forward: GTCTCTTGCGGTTATTACGCCTT - Reverse: CACGAAATTGGGATCGGCGTTAG HCN2-4 outer Forward: GTGGGCATCACTTTCTTCAAGGA - Reverse: CGGGCAGCTTGTGGAAGGACATGTA HCN1 inner Forward: CGCCTTTCAAGGTTAATCAGATA 404 Reverse: CTTGAAGAGTCCAAAGACTGG HCN2 inner Forward: TCCGCACCGGCATTGTTATT 658 Reverse: TGCTTGTACTTCTCCTGGTA HCN3 inner Forward: CGCCAAGGGCCATCCGAACGCGT 619 Reverse: TGCTTGTACTTCTCCTGGTA HCN4 inner Forward: TCAAGATGAAGTACCTGAAA 604 Reverse: TGCTTGTACTTCTCCTGGTA |