Table 1 of
Koizumi, Mol Vis 2004;
10:328-340.Table 1 of
Koizumi, Mol Vis 2004;
10:328-340.
Table 1. RT-PCR primers
The outer primers were used for reverse transcription and the first-round PCR. A common outer primer pair was used to amplify mRNAs for HCN2-4. The inner primers were used for the nested second-round PCR. HCN2-4 were amplified using gene specific forward primers and a common reverse primer.
Size
Primer name Primer sequence (bp)
------------ ---------------------------------- ----
HCN1 outer Forward: GTCTCTTGCGGTTATTACGCCTT -
Reverse: CACGAAATTGGGATCGGCGTTAG
HCN2-4 outer Forward: GTGGGCATCACTTTCTTCAAGGA -
Reverse: CGGGCAGCTTGTGGAAGGACATGTA
HCN1 inner Forward: CGCCTTTCAAGGTTAATCAGATA 404
Reverse: CTTGAAGAGTCCAAAGACTGG
HCN2 inner Forward: TCCGCACCGGCATTGTTATT 658
Reverse: TGCTTGTACTTCTCCTGGTA
HCN3 inner Forward: CGCCAAGGGCCATCCGAACGCGT 619
Reverse: TGCTTGTACTTCTCCTGGTA
HCN4 inner Forward: TCAAGATGAAGTACCTGAAA 604
Reverse: TGCTTGTACTTCTCCTGGTA
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