Figure 5 of
Koizumi, Mol Vis 2004;
Figure 5. ZD7288 partially suppresses IIR
IIR was partially suppressed by ZD7288, a blocker of HCN channels. A: Voltage-clamp experiment in the control condition. Voltage steps were from -111 mV to -31 mV, in 10 mV steps, for 200 msec. B: The same voltage-clamp experiment as in A with ZD7288 (100 μM) in the extracellular solution. C: The I-V relationship in the sustained state for panels A and B. Filled circles, control; Open squares, ZD7288. D: Current-clamp experiment in the control condition. Injected currents were from -40 pA to 40 pA, in 20 pA steps, for 200 msec. Input resistance of this cell was calculated as 533 MΩ. Oscillatory potentials are indicated by an arrow. E: The same current clamp experiment as in panel D with ZD7288 (100 μM) in the extracellular solution. Input resistance of this cell was increased to 739 MΩ. Oscillatory potentials are indicated by an arrow. F: Line plots for all six cells examined. G: Maximum conductances of IIR. External Cs+ suppresd IIR to 38±21% (n=9), and ZD7288 suppressed it to 52±20% (n=6).