Figure 1 of
Mukhopadhyay, Mol Vis 2004;
10:304-314.Figure 1 of
Mukhopadhyay, Mol Vis 2004;
10:304-314.
Figure 1. Comparison of the primary sequence of noelin 1_v1, noelin 2_v1, noelin 3_v1, and myocilin
The comparison between protein sequences was done using the ClustalW alignment tool. Between these sequences the identical, conserved, and semi-conserved residues are represented by asterisk, colon, and single dots, respectively. The locations of the splice junctions in the genes are marked in red. Inter-codon and intra-codon splice junctions are indicated by marking two letters and a single letter, respectively. Within three Noelin gene products all splice junctions, excepting the first one, were conserved. The location of the second and last splice junction of Myocilin coincides with the fifth splice junctions of the noelin splice variants shown in the illustration.
Noelin l_v1 ----MPGRWRWQRDMHPARKLLSLLFLILMGTELTQVLPTNPEESWQVYSSAQDSEGRCI 56
Noelin 3_v1 --------------MQATSNLLNLLLLSLFAGLDPSKTQISPKEGWQVYSSAQDPDGRCI 46
Noelin 2_v1 --------------MWPLT-VPPPLLLLLCSGLAGQTLFQNPEEGWQLYTSAQAPDGKCI 45
Myocilin MRFFCARCCSFGPEMPAVQLLLLACLVWDVGARTAQLREANDQSGRCQYTFSVASPNESS 60
* . : :: . . . :.. *: : . ...
Noelin l_v1 CTVVAPQQTMCS------------RDARTKQLRQLLEKVQNMSQSIEVLDRRTQRDLQYV 104
Noelin 3_v1 CTVVAPEQNLCS------------RDAKSRQLRQLLEKVQNMSQSIEVLNLRTQRDFQYV 94
Noelin 2_v1 CTAVIPAQSTCS------------RDGRSRELRQLMEKVQNVSQSMEVLELRTYRDLQYV 93
Myocilin CPEQSQAMSVIHNLQRDSSTQRLDLEATKARLSSLESLLHQLTLDQAARPQETQEGLQRE 120
*. :. . .* .* . ::::: . . .* ..:*
Noelin l_v1 EKMENQMKG-LESKFKQVEESHKQHLARQFKAIKAKMDELRPLIPVLEEYKADAKLVLQF 163
Noelin 3_v1 LKMETQMKG-LKAKFRQIEDDRKTLMTKHFQELKEKMDELLPLIPVLEQYKTDAKLITQF 153
Noelin 2_v1 RGMETLMRS-LDARLRAADGS---LSAKSFQELKDRMTELLPLSSVLEQYKADTRTIVRL 149
Myocilin LGTLRRERDQLETQTRELETAYSNLLRDKSVLEEEKKRLRQENENLARRLESSSQEVARL 180
:. *.:: : : : : : .. ::.:: : ::
Noelin l_v1 K----EEVQNLTSVLNELQEEIGAY-----DYDELQSRVSNLEER----------LRACM 204
Noelin 3_v1 K----EEIRNLSAVLTGIQEEIGAY-----DYEELHQRVLSLETR----------LRDCM 194
Noelin 2_v1 R----EEVRNLSGSLAAIQEEMGAY-----GYEDLQQRVMALEAR----------LHACA 190
Myocilin RRGQCPQTRDTARAVPPGSREVSTWNLDTLAFQELKSELTEVPASRILKESPSGYLRSGE 240
: : :: : : ..*:.:: :::*:..: : *:
Noelin l_v1 QKLACGKLTGISDPVTVKTSG---SRFGSWMTDP--LAPEGDNRVWYMDGY-HNNRFVRE 258
Noelin 3_v1 KKLTCGKLMKITGPVTVKTSG---TRFGAWMTDP--LASEKNNRVWYMDSY-TNNKIVRE 248
Noelin 2_v1 QKLGCGKLTGVSNPITVRAMG---SRFGSWMTDT--MAPSADSRVWYMDGY-YKGRRVLE 244
Myocilin GDTGCGELVWVGEPLTLRTAETITGKYGVWMRDPKPTYPYTQETTWRIDTVGTDVRQVFE 300
. **:* : *:*::: ::* ** *. . :. .* :* . : * *
Noelin l_v1 YKSMVDFMNTDNFTSHRLPHPWSGTGQVVYNGSIYFNKFQSHIIIRFDLKTETILKTRSL 318
Noelin 3_v1 YKSIADFVSGAESRTYNLPFKWAGTNHVVYNGSLYFNKYQSNIIIKYSFDMGRVLAQRSL 308
Noelin 2_v1 FRTLGDFIKGQNFIQHLLPQPWAGTGHVVYNGSLFYNKYQSNVVVKYHFRSRSVLVQRLS 304
Myocilin YDLISQFMQGYPSKVHILPRPLESTGAVVYSGSLYFQGAESRTVIRYELNTETVEAEKEI 360
: : :*:. : ** .*. ***.**:::: :*. :::: : : : :
Noelin l_v1 DYAGYNNMYHYAWGGHSDIDLMVDESGLWAVYATNQNAGNIVVSRLDPVSLQTLQTWNTS 378
Noelin 3_v1 EYAGFHNVYPYTWGGFSDIDLMADEIGLWAVYATNQNAGNIVISQLNQDTLEVMKSWSTG 368
Noelin 2_v1 PGAGYNNTFPYSWGGFSDMDFMVDESGLWAVYTTNQNAGNIVVSRLDPHTLEVMRSWDTG 364
Myocilin PGAGYHGQFPYSWGGYTDIDLAVDEAGLWVIYSTDEAKGAIVLSKLNPENLELEQTWETN 420
**::. : *:***.:*:*: .** ***.:*:*:: * **:*:*: .*: ::*.*.
Noelin l_v1 YPKRSAGEAFIICGTLYVTNGYSGG-TKVHYAYQTNASTYEYIDIPFQNKYSHISMLDYN 437
Noelin 3_v1 YPKRSAGESFMICGTLYVTNSHLTG-AKVYYSYSTKTSTYEYTDIPFHNQYFHISMLDYN 427
Noelin 2_v1 YPKRSAGEAFMICGVLYVTNSHLAG-AKVYFAYFTNTSSYEYTDVPFHNQYSHISMLDYN 423
Myocilin IRKQSVANAFIICGTLYTVSSYTSADATVNFAYDTGTGISKTLTIPFKNRYKYSSMIDYN 480
*:*..::*:***.**....: . :.* ::* * :. : :**:*:* : **:***
Noelin l_v1 PKDRALYAWNNGHQILYNVTLFHVIRSDEL- 467
Noelin 3_v1 ARDRALYAWNNGHQVLFNVTLFHIIKTEDDT 458
Noelin 2_v1 PRERALYTWNNGHQVLYNVTLFHVISTSGDP 454
Myocilin PLEKKLFAWDNLNMVTYDIKLSKM------- 504
. :: *::*:* : : :::.* ::
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