Figure 1. Phosphorylation of Trk A and Trk C receptors following treatment with NGF or NT-3

Cells were treated with NT, pre-treated with K-252a and then treated with NT, or treated with K-252a alone. Untreated LC cells and ONH astrocytes served as controls. Immunoprecipitated Trk proteins were blotted with an anti-phospho-pan-Trk antibody (pTrk), stripped, and re-probed with anti-Trk A or anti-Trk C antibody. The terms neurotrophin (NT), NGF treated (NGF T), NT-3 treated (NT-3 T), and immunoprecipitation (IP) are abbreviated in the figure.