Figure 2. SIN-1 treatment increases mRNA levels of TIMP-1 and MMP-2

Normal stromal fibroblasts were cultured with or without 1 mM SIN-1 or 1 mM SNAP for 18 h. RNA was harvested from each culture and analyzed by northern analysis with probes generated by random priming the cDNA of TIMP-1, MMP-2, or β-actin. Signals for each hybridized probe were obtained with phosphorimaging and the TIMP-1 and MMP-2 normalized by comparison to β-actin. SIN-1 treated cultures showed significant increased of mRNA levels for both TIMP-1 and MMP-2 (p<0.01). SNAP treated cultures had mRNA levels similar to untreated.