Figure 1. SIN-1 leads to nitrotyrosine accumulation

Cultures of normal stromal cells were cultured with or without either 1 mM SIN-1 or 1 mM SNAP. The cells were harvested at 4 h, 8 h, or 24 h and investigated by western analysis using a monoclonal antibody to nitrotyrosine. The secondary antibody alone shows some background staining (not shown). A prominently stained band in the SIN-1 treated cell cultures that migrated near the 64 kDa marker was noted.