Table 1 of
Pina, Mol Vis 2004;
10:265-271.Table 1 of
Pina, Mol Vis 2004;
10:265-271.
Table 1. PCR primers used for genomic amplification of the exons of human GBT2
Shown are the gene-specific sequences for the forward (f) and reverse (r) primers, their annealing temperatures, and their location within the α-cone transducin gene. The sequence numbering is from GenBank accession number Z18859.
Annealing
Exon Primer Sequence Temp (°C) Location
---- ------------------------------- --------- ---------
1 f: 5'-AGGAAACCTTACGAGAGAGGATTAG 66 159-183
r: 5'-CAGGAAAGTAGGAAGGACCCCTGCC 362-386
2 f: 5'-TGGAAGGCTGAGGAGAACTGGTGGA 66 2521-2545
r: 5'-GGCTGTACTGACTTCTTCACCCTAT 2649-2673
3 f: 5'-CCCTTAGGTCTGGTTACTCAGGTCC 66 2846-2870
r: 5'-TCTTATTGCTAGCCTTTCTGCTTCC 3073-3097
4 f: 5'-CCTCCTGGAAAGCTATTACTCCTGT 68 4239-4263
r: 5'-GACTGGCTAGAAGATTGCTTAAGCA 4482-4506
5 f: 5'-TCTCTTAGCCTCGTCTGTGGAAAAT 58 6592-6616
r: 5'-CCTTTTCAGGATTCCACACGTGTAT 6806-6830
6 f: 5'-GGCATATAGGTCGTATGTTGGGCAT 68 6929-6953
r: 5'-AGGGTCATGGGGAGAACTTGTTCTA 7144-7168
7 f: 5'-GGTAGATATTCTATAAGCCAAATCT 62 8924-8948
r: 5'-AATTGCCAGTCTCTACTAAAAGGCA 9163-9187
8 f: 5'-GCAACTAACAAGGGTTCATTTATTC 62 9484-9508
r: 5'-AATTACCCAGATTCCAAGCCTGTTT 9760-9784
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