Figure 5. Apoptosis induced by fusion protein expression

TUNEL assay of transfected HEK293 cells expressing EGFP/wtELOVL4 (WT) or EGFP/mtELOVL4 (MT) fusion proteins. The TUNEL assay of vector control (VC) is co-plotted. Assays were performed after 20 h of transfection. After a TUNEL reaction, cells were observed using episcopic-fluorescence microscope. The green channel was used to detect fusion protein expression and red channel was used to detect apoptotic nuclear staining. Results were plotted as percent apoptotic cells per equivalent number of transfected cells.