Table 3 of
McKay, Mol Vis 2004;
10:240-247.Table 3 of
McKay, Mol Vis 2004;
10:240-247.
Table 3. Analysis of mass spectrometry data
When peak list files were submitted using Mascot to an MS/MS Ion Search of MSDB, a number of proteins were identified in each retinal preparation. In addition the sequence of 44 peptides was determined by AutoMod de novo sequencing; several of these were matched by BLAST searching to proteins in SWISSPROT (name and accession number given in parentheses).
Proteins identified by
Preparation Mascot search of MSDB (Accession) De novo sequenced peptides (Swissprot)
----------- --------------------------------------------- -------------------------------------------
GCL Hemoglobin beta chain (HBPG) QDTGPFPFQYKN
Glutathione S-Transferase (GTP-PIG) LNETGDQPVDPGAEL
Glutamine synthetase (A3406) KDVAATVPSAGPVLPCPFPVNLTEVLGGGVGVLT
Alpha enolase (ENOA_BOVIN) TSVLAARRLAKS
Glial fibrillary acidic protein (A35734) YSVSSLELAPVF
VHLSAETSPTAVLGL
VDEVLGGSLGAVV
TRGAVVGAAKSPTLCPEFADKPPFAALKGPL
VAYYYVAYVYELEYLAQAATVAEVVEEGALLELVGAAGALLA
NKPLDKKF
LVVYPEGTKVFG
FEEEIKAEQEER (Swiprosin 1, Q9D8Y0)
INL H3 histone (BAC56360) LNETGDPPPAGTGSLE
S-antigen (AAB46757) TKAPSSPPKAGAALVLAGTAAGL
Hemoglobin beta chain (HBPG) TQPAAAEAGLAGLAASTSKP
LQSAGALLSSPSLNPVQPSVNVTKCGPKSGAGPL
LDDLVPAAGK
KEDAGVTPPEPLKDAADLKEEEEGAPQLQEV
KPDTTLPSAELPNKDDALPQFPLDLLPEQLASGAQAAP
AAPLTTALR
EKPFSAVPPMPRPPPGQLAGKAYLSGLA
PRL S-antigen (AAB46757) ARETGPPAGTKDLEL
Glutamine synthetase (A34006) KTVLGVLAGAPAPPKKVTAPKGKL
Histone H3 (BAC56329) LGSALPKKAAT
Cytochrome C Oxidase (PN0607) ELLAAEKSLKFLKEEKQLLNNSGLL
KTVLGVLAGAPAPPKKVTAPKGKL
EVDLVPAKK
QRAAAEKASVSEEKLLTPVTKLALQ
RTAGLLVKTLLPSLPLKKSPPKKVTLQ
FTVLVKLTLVVKN
TLPLGKSVGALLSL
WR pyruvate kinase (1AQFE) TRGGPAGSPFLVGGGGAGGKV
Alpha enolase (ENOA_BOVIN) VVYPWTKRF
S-antigen (AAB46757) LVVYPDATKRF
70 kDa heat shock protein HSP70 (BAC57466) PVDLGSKVGSLPSL
27 kDa Heat shock protein HSP60 (Q8VEF4) AYLCVLDAYQLAWYYVALALQYTDAVGLGGLYAYVGLCVCLFA
Glutamine synthetase (AAO64254) PVGAPSGGLPGPA
Glutathione S-Transferase (GTP-PIG) LNETGDKPDPPEAAT
mitochondrial malate dehydrogenase (CAA30274) RDTPLFRFEGSNLLNQRVT
RLSPDRLY
KNLLSPLVLSALSV
PPYTLTPVNRPVGEPAKPGAVGSLP
VIQKNLVYVVGINPPVPYEEVAPTLK (General
negative regulator of transcription
subunit 4, P34909)
FEEEIKAEQEER (Swiprosin 1, Q9D8Y0)
|