Table 2 of
McKay, Mol Vis 2004;
10:240-247.Table 2 of
McKay, Mol Vis 2004;
10:240-247.
Table 2. PCR primers used in amplification of porcine retinal cDNA
The porcine sequence was not available for the neurofilament gene and primers were therefore designed from conserved regions of orthologous genes from other species; the accession numbers are provided for the human, mouse, and rat orthologs. The accession number provided for the 18S and 28S genes is from mouse, however the primer sequences also match highly conserved regions within rat and human.
Forward (top) and reverse Product
Gene GenBank accession number (bottom) primer sequences size (bp)
---------------- ----------------------------- ------------------------------ ---------
Rhodopsin AF008947 CAT GAC CAT CCC AGC CTT CTT 151
AAG TGG TGG TGG AGG CCT CGT
Cone opsin AY091587 ATG AGC AAG ATG CCA GAG GAG G 180
CAC TCA ATG CCA CGG TTC TGG TG
Glial fibrillary AJ551395 GAT GAA ACC AAC CTG AGG CTG G 185
acidic protein TGC TGT GCC AGC TGC TCC T
S-antigen S82664 ATG GCA GCC AAC GTG CAG CGC 201
AGG TCA GAG AGA CGT ACA CTC T
Neurofilament NM_005382 (Homo sapiens), AGG CCG AAG AGT GGT TCA A 316
X02165 (Mus musculus), ATC TTG ACG TTG AGG AGG T
NM_031783 (Rattus norvegicus)
18S BK000964 GTA ACC CGT TGA ACC CCA TT 151
CCA TCC AAT CGG TAG TAG CG
28S BK000964 TTG AAA ATC CGG GGG AGA G 100
ACA TTG TTC CAA CAT GCC AG
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