Table 2 of McKay, Mol Vis 2004; 10:240-247.

Table 2. PCR primers used in amplification of porcine retinal cDNA

The porcine sequence was not available for the neurofilament gene and primers were therefore designed from conserved regions of orthologous genes from other species; the accession numbers are provided for the human, mouse, and rat orthologs. The accession number provided for the 18S and 28S genes is from mouse, however the primer sequences also match highly conserved regions within rat and human.

                                                     Forward (top) and reverse       Product
      Gene           GenBank accession number        (bottom) primer sequences      size (bp)
----------------   -----------------------------   ------------------------------   ---------
Rhodopsin          AF008947                        CAT GAC CAT CCC AGC CTT CTT         151
                                                   AAG TGG TGG TGG AGG CCT CGT

Cone opsin         AY091587                        ATG AGC AAG ATG CCA GAG GAG G       180
                                                   CAC TCA ATG CCA CGG TTC TGG TG

Glial fibrillary   AJ551395                        GAT GAA ACC AAC CTG AGG CTG G       185
acidic protein                                     TGC TGT GCC AGC TGC TCC T

S-antigen          S82664                          ATG GCA GCC AAC GTG CAG CGC         201
                                                   AGG TCA GAG AGA CGT ACA CTC T

Neurofilament      NM_005382 (Homo sapiens),       AGG CCG AAG AGT GGT TCA A           316
                   X02165 (Mus musculus),          ATC TTG ACG TTG AGG AGG T
                   NM_031783 (Rattus norvegicus)

18S                BK000964                        GTA ACC CGT TGA ACC CCA TT          151
                                                   CCA TCC AAT CGG TAG TAG CG

28S                BK000964                        TTG AAA ATC CGG GGG AGA G           100
                                                   ACA TTG TTC CAA CAT GCC AG

McKay, Mol Vis 2004; 10:240-247 <>
©2004 Molecular Vision <>
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