Table 1 of
Kijas, Mol Vis 2004;
10:223-232.Table 1 of
Kijas, Mol Vis 2004;
10:223-232.
Table 1. Primers used for the RT-PCR based cloning of the canine ABCA4 gene
Nine pairs of canine specific primers were designed to amplify overlapping fragments spanning the 6810 bp ABCA4 translated region. Primers were designed based on Genbank entries AY427777, AY427778, and AY427779.
STS
Primer Sequence (5'-3') bp Exons
---------------- ------------------------ ---- --------
17B ATGTCCTTTCCTGTCGCTGCACC 597 5'UTR-4
18 CAACTGAGTCAGACAGGCCAATGT
1 ACCTTGTCTCGACTCATGGACACC 1053 4-11
2 GAAGTTCAGCATGGCTTCTGCAG
3a CACAGATGACCATGATCAGAGAC 1197 11-16
4a GGAGCATCATCTTCATGGACATC
5 TTGGCACTGAGTACCTGGCTCGCT 1077 16-23
6 TCTTGCGCACCAAGGTTAAG
7 TGTCCACTCACCACATGGACGA 1402 23-33
8b ATCTGGCCAAGGTGGCTTAA
9 GAGCAAATTCTGGGTCAAT 796 32-38
10 GATGAAGGTGATGGCACTGCTG
11 CAGCACAGCCTATGTGGCCTTG 623 38-43
12 CGTCACCTGAGGTCACTGTGGT
13 GTCTTCTGGGAGTGAATGGAGCT 572 43-47
14 GACTTGAGATGCTGAATGGTGC
15 GAGGAGTGTGAGGCTCTGTGTA 522 47-3'UTR
16A GGTCCAATCAGATGACCATAT
ABCA4-ex13SNPFor CTGATCCTGTGGAAGACTTCC 134 exon 13
ABCA4-ex13SNPRev GTAAGGCATCTGCTGGAGATAG
ABCA4-ex27SNPFor CAGGGCTCCAATGGCTGCTCT 146 exon 27
ABCA4-ex27SNPRev TGGTGGAATCGCTTGACCAGCAA
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