Figure 1 of
Han, Mol Vis 2004;
10:215-222.Figure 1 of
Han, Mol Vis 2004;
10:215-222.
Figure 1. cDNA encoding guinea pig aquaporin 0 and its deduced 263 amino acid sequence
The 789 bp cDNA (Genbank accession number AY485151) contained no untranslated 5' or 3' sequences and was missing its stop codon, since it was included in the downstream PCR primer. Nucleotides at positions 161, 221, and 383 were different in the 2 sequenced clones (A/C=M, C/T=Y, G/A=R). This resulted in three questionable residues at position 54 (T or K), 74 (A or V) and 128 (G or D).
ATGTGGGAACTGCGATCTGCCTCCTTCTGGAGGGCCATATTTGCTGAGTTCTTTGCTACC 60
M W E L R S A S F W R A I F A E F F A T
10 20
CTTTTCTATGTCTTCTTTGGGCTTGGGGCCTCACTGCGCTGGGCCCCCGGACCCCTGCAT 120
L F Y V F F G L G A S L R W A P G P L H
30 40
GTCCTGCAGGTGGCTTTGGCCTTTGGCTTGGCCCTGGCTAMATTGGTGCAGACAGTGGGC 180
V L Q V A L A F G L A L A X L V Q T V G
50 60
CACATCAGCGGAGCCCACGTCAATCCTGCAGTGACTTTTGYCTTCCTTGTGGGCTCCCAG 240
H I S G A H V N P A V T F X F L V G S Q
70 80
ATGTCCCTGCTCCGAGCCTTCTGTTATATGGCAGCCCAGCTCCTGGGGGCTGTGGCTGGG 300
M S L L R A F C Y M A A Q L L G A V A G
90 100
GCTGCTGTACTGTACAGTGTTACCCCACCTGCTGTCCGAGGAAACCTCGCACTCAACACG 360
A A V L Y S V T P P A V R G N L A L N T
110 120
TTACACGCTGGGGTGAGTGTTGRCCAGGCCACCACAGTGGAGATCTTCTTGACACTCCAG 420
L H A G V S V X Q A T T V E I F L T L Q
130 140
TTCGTGCTCTGCATCTTTGCCACATACGATGAGAGGCGAAACGGCCGCCTGGGCTCTGTT 480
F V L C I F A T Y D E R R N G R L G S V
150 160
GCTCTAGCTGTTGGCTTCTCCCTCACCTTGGGACACCTTTTTGGGATGTACTATACTGGT 540
A L A V G F S L T L G H L F G M Y Y T G
170 180
GCAGGCATGAATCCTGCCCGCTCCTTTGCTCCTGCCATTCTTACTCGGAACTTCACCAAC 600
A G M N P A R S F A P A I L T R N F T N
190 200
CACTGGGTGTACTGGGTGGGCCCAATCATTGGAGGGGGTCTGGGCAGCCTTCTCTACGAC 660
H W V Y W V G P I I G G G L G S L L Y D
210 220
TTTCTGCTCTTCCCCCGGCTCAAGAGTGTTTCTGAGAGACTGTCTATTCTCAAGGGTACC 720
F L L F P R L K S V S E R L S I L K G T
230 240
AGACCCAGTGACAACAATGGACAACCAGAGGGCACTGGGGAACCTGTTGAACTGAAGACC 780
R P S D N N G Q P E G T G E P V E L K T
250 260
CAGGCCCTG 789
Q A L
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