Table 1 of
Zhang, Mol Vis 2004;
10:208-214.Table 1 of
Zhang, Mol Vis 2004;
10:208-214.
Table 1. DNA constructs used in the transgene expression system
pLuc series contain the 5x17-mer GAL4 binding site and the luciferase reporter gene (luc) regulated by the different promoters; pGV series contain GAL4-VP16 DNA regulated by CMVP or R65P (Figure 1A). pLuc-GV series combine luc, the 5x17-mer and GAL4-VP16 DNAs in the same construct (Figure 1B). There are two types of single DNAs according to the regulation of the reporter gene: the minimal promoter (TATA) or the RPE65 promoter (R65P).
Promoter regulating
the reporter gene
Name (luc)/GAL4-VP16 (GV) Comments
-------------- -------------------- -----------------------------------------------
Double DNA pLuc series
pMluc (pG5luc) TATA-luc
pCMVluc CMVP-luc
phR65luc hR65P-luc Transfection or co-transfection with pGV series
pmR65luc mR65P-luc
Double DNA pGV series
pCMVGV CMVP-GV
phR65GV hR65P-GV Co-transfection with pLuc series
pmR65GV mR65P-GV
Single DNA pLuc-GV series
pMluc-CMVGV TATA-luc CMVP-GV
pMluc-hR65GV TATA-luc hR65P-GV
pMluc-mR65GV TATA-luc mR65P-GV Transfection with a pLuc-GV DNA
phR65luc-GV hR65P-luc hR65P-GV
pmR65luc-GV mR65P-luc mR65P-GV
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