Figure 2 of
Zhang, Mol Vis 2004;
Figure 2. Schematic illustration of DNA constructs and reporter activities
Schematic diagram of DNA constructs in the system and reporter activities of different DNA constructs in RPE51 cells. The cells were transiently transfected or co-transfected with the different DNA constructs and incubated for 40 h prior to luciferase assay. A: pLuc constructs and their promoter activities. B: pGV constructs and enhancement of the TATA activity in pMluc co-transfection with pGV, correspondingly. C: pR65luc, pR65GV constructs and enhancement of the RPE65 promoter activity in pR65luc co-transfection with pR65GV, correspondingly. D: pLuc-GV constructs and activities of the single plasmids transfection. The luciferase activities, relative light unit (RLU) readings from the luminometer, were evaluated as fold relative to the activity of pCMVluc (CMVP=100). The error bars represent the standard deviations of triplicate measurements.