Figure 3 of
Rundle, Mol Vis 2004;
10:177-185.Figure 3 of
Rundle, Mol Vis 2004;
10:177-185.
Figure 3. Amino acid sequence of Type II NMTs
bNMT II (bNMT) retina is from bovine retina (GenBank accession number AF222687), bNMT1 II (bNMT1) is bovine liver from library screening (GenBank accession number AY007993), mNMT II (mNMT) and hNMT II (hNMT) are from mouse and human liver, respectively [8], and bNMT2 (bNMT2) is bovine liver Type II found by PCR. Red lettering indicates amino acid differences and blue indicates similarity. Multiple sequence alignment by clustalw and Boxshade at Genestream, Institute de Génétique Humaine.
bNMT retina 0 MAEDSESAASQQSLELDDQDTCGIDGDNEEETEHAKGSPGGDLGAKKKKKKQKRKKEKP bNMT1 liver -----------------------------------------------------RKKEKP mNMT liver 0 MAEDSESAASQQSLELDDQDTCGIDGDNEEETEHAKGSPGGDLGAKKKKKKQKRKKEKP bNMT2 liver 0 MAEDSESAASQQSLELDDQDTCGIDGDNEEETEHAKGSPGGYLGAKKKKKKQKRKKEKP hNMT liver 0 MAEDSESAASQQSLELDDQDTCGIDGDNEEETEHAKGSPGGYLGAKKKKKKQKRKKEKP bNMT retina 60 NSGGTKSDSASDSQEIKIQPPS-------------------------------KNSTIPV bNMT1 liver 7 NSGGTKSDSASDSQEIKIQPPS-------------------------------KNSTIPV mNMT liver 60 NSGGTKSDSASDSQEIKIQQSSKHNAIWQQISAGAAMGGDTMEGEWIDLRMYHKNPTIPI bNMT2 liver 60 NSGGTKSDSASDSQEIKIQQPS-------------------------------KNPSVPM Hnmt liver 60 NSGGTKSDSASDSQEIKIQQPS-------------------------------KNPSVPM bNMT retina 89 QKLQDIQRAMELLSACQGPARNIDEAAKHRYQFWDTQPVPKLNEVITSHGAIEADKENVR bNMT1 liver 36 QKLQDIQRAMELLSACQGPARNIDEAAKHRYQFWDTQPVPKLNEVITSHGAIEADKENVR mNMT liver 120 QKLQDIQRAMELLSACQGPARNIDEATKRRYQFWDTQPVPKLNEVITSHGAIEPDKDNIR bNMT2 liver 89 QKLQDIQRAMELLSACQGPARNIDEAAKHRYQFWDTQPVPKLDEVITSHGAIEPDKDNVR hNMT liver 89 QKLQDIQRAMELLSACQGPARNIDEAAKHRYQFWDTQPVPKLDEVITSHGAIEPDKDNVR bNMT retina 148 QEPYSLPQGFMWDTLDLGNAEVLRELYTLLNENYVEDDDNMFRFDYSPEFLLWALRPPGW bNMT1 liver 96 QEPYSLPQGFMWDTLDLGNAEVLRELYTLLNENYVEDDDNMFRFDYSPEFLLWALRPPGW mNMT liver 180 QEPYSLPQGFMWDTLDLSNAEVLKELYTLLNENYVEDDDNMFRFDYSPEFLLWALRPPGW bNMT2 liver 148 QEPYSLPQGFMWDTLDLSDAEVLKELYTLLNENYVEDDDNMFRFDYSPEFLLWALRPPGW hNMT liver 148 QEPYSLPQGFMWDTLDLSDAEVLKELYTLLNENYVEDDDNMFRFDYSPEFLLWALRPPGW bNMT retina 207 LLQWHCGVRVSSNKKLVGFISAIPANIRIYDSVKKMVEINFLCVHKKLRSKRVAPVLIRE bNMT1 liver 156 LLQWHCGVRVSSNKKLVGFISAIPANIRIYDSVKKMVEINFLCVHKKLRSKRVAPVLIRE mNMT liver 239 LLQWHCGVRVSSNKKLVGFISAIPANIRIYDSVKRMVEINFLCVHKKLRSKRVAPVLIRE bNMT2 liver 207 LLQWHCGVRVSSNKKLVGFISAIPANIRIYDSVKKMVEINFLCVHKKLRSKRVAPVLIRE hNMT liver 207 LLQWHCGVRVSSNKKLVGFISAIPANIRIYDSVKKMVEINFLCVHKKLRSKRVAPVLIRE bNMT retina 256 ITRRVNLEGIFQAVYTAGVVLPKPVATCRYWHRSLNPRKLVEVKFSHLSRNMTLQRTMKL bNMT1 liver 216 ITRRVNLEGIFQAVYTAGVVLPKPVATCRYWHRSLNPRKLVEVKFSHLSRNMTLQRTMKL mNMT liver 298 ITRRVNLEGIFQAVYTAGVVLPKPVATCRYWHRSLNPRKLVEVKFSHLSRNMTLQRTMKL bNMT2 liver 256 ITRRVNLEGIFQAVYTAGVVLPKPIATCRYWHRSLNPRKLVEVKFSHLSRNMTLQRTMKL hNMT liver 256 ITRRVNLEGIFQAVYTAGVVLPKPIATCRYWHRSLNPRKLVEVKFSHLSRNMTLQRTMKL bNMT retina 315 YRLPDATKTSGLRPMEPRDIKAVQELTNTYLKQFHLAPVMDEEEVAHWFLPQEHIIDTFV bNMT1 liver 276 YRLPDATKTSGLRPMEPRDIKAVQELTNTYLKQFHLAPVMDEEEVAHWFLPQEHIIDTFV mNMT liver 357 YRLPDVTKTSGLRPMEPKDIKAVRELINIYLKQFHLAPVMDDAEVAHWFLPREHIIDTFV bNMT2 liver 315 YRLPDVTKTSGLRPMEPKDIKSVRELINTYLKQFHLAPVMDEEEVAHWFLPREHIIDTFV hNMT liver 315 YRLPDVTKTSGLRPMEPKDIKSVRELINTYLKQFHLAPVMDEEEVAHWFLPREHIIDTFV bNMT retina 374 VENSSGKLTDFLSFYTLPSTVMHHPAHKSLKAAYSFYNIHTETPLLDLMSDALIIAKLKG bNMT liver 336 VENSSGKLTDFLSFYTLPSTVMHHPAHKSLKAAYSFYNIHTETPLLDLMSDALIIAKLKG mNMT liver 416 VENPSGKLTDFLSFYTLPSTVMHHPAHKSLKAAYSFYNIHTETPLLDLMNDALIIAKLKG bNMT2 liver 374 VESPNGKLTDFLSFYTLPSTVMHHPAHKSLKAAYSFYNIHTETPLLDLMSDALILAKSKG hNMT liver 374 VESPNGKLTDFLSFYTLPSTVMHHPAHKSLKAAYSFYNIHTETPLLDLMSDALILAKSKG bNMT retina 433 FDVFNALDLMENKTFLEKLKFGIGDGNLQYYLYNWRCPGTESEKVGLVLQ* bNMT1 liver 396 FDVFNALDLMENKTFLEKLKFGIGDGNLQYYLYNWRCPGTESEKVGLVLQ* mNMT liver 475 FDVFNALDLMENKTFLEKLKFGIGDGNLQYYLYNWRCPGTDSEKVGLVLQ* bNMT2 liver 433 FDVFNALDLMENKTFLEKLKFGIGDGNLQYYLYNWRCPGTDSEKVGLVLQ* hNMT liver 433 FDVFNALDLMENKTFLEKLKFGIGDGNLQYYLYNWRCPGTDSEKVGLVLQ* |