Figure 1 of
Rundle, Mol Vis 2004;
10:177-185.Figure 1 of
Rundle, Mol Vis 2004;
10:177-185.
Figure 1. Amino acid sequence of Type I NMTs
Long form hNMT I human muscle and hNMT I human liver (GenBank accession numbers AAH08579 and AF043324), short form hNMTS represents human HepG2 cells [6], bNMTS I sp/ret is the short form of bovine spleen [9] and bovine retina (GenBank accession number AF223384), and mouse mNMT I liver (GenBank accession number AF043326) amino acid alignment. Red lettering indicates amino acid difference, blue indicates similarity, and green indicates conserved amino acid changes. Multiple sequence alignment was by clustalw and Boxshade at Genestream, Institute de Génétique Humaine.
hNMT I muscle 1 MADESETAVKPPAPPLPQMMEGNGNGHEHCSDCENEEDNSYNRGGLSPANDTGAKKKKK hNMT I liver 1 MADESETAVKPPAPPLPQMMEGNGNGHEHCSDCENEEDNSYNRGGLSPANDTGAKKKKK hNMTS I HepG2 0 ----------------------------------------------------------- bNMT I retina 1 MGDESETAVKPPAPPLPQMMEGNGNGHEHCSDCENEEDNSYNRGGLSPANDTGAKKKKK bNMTS I sp/ret 0 ----------------------------------------------------------- mNMT I liver 1 MADESETAVKLPAPSLPLMMEGNGNGHEHCSDCENEEDNSHNRSGLSPANDTGAKKKKK hNMT I muscle 61 KQKKKKEKGSETDSAQDQPVKMNSLPAERIQEIQKAIELFSVGQGPAKTMEEASKRSYQ hNMT I liver 61 KQKKKKEKGSETDSAQDQPVKMNSLPAERIQEIQKAIELFSVGQGPAKTMEEASKRSYQ hNMTS I HepG2 1 ---------------------MNSLPAERIQEIQKAIELFSVGQGPAKTMEEASKRSYQ bNMT I retina 61 KQKKKKEKGSETDSAQDQPVKMNSLPAERIQEIQKAIELFSVGQGPAKTMEEASKRSYQ bNMTS I sp/ret 1 ---------------------MNSLPAERIQEIQKAIELFSVGQGPAKTMEEASKRSYQ mNMT I liver 61 KQKKKKEKGSDMESTQDQPVKMTSLPAERIQEIQKAIELFSVGQGPAKTMEEASKRSYQ hNMT I muscle 120 FWDTQPVPKLGEVVNTHGPVEPDKDNIRQEPYTLPQGFTWDALDLGDRGVLKELYTLLNE hNMT I liver 120 FWDTQPVPKLGEVVNTHGPVEPDKDNIRQEPYTLPQGFTWDALDLGDRGVLKELYTLLNE hNMTS I HepG2 40 FWDTQPVPKLGEVVNTHGPVEPDKDNIRQEPYTLPQGFTWDALDLGDRGVLKELYTLLNE bNMT I retina 120 FWDTQPVPKLGEVVNTHGPVEPDKDNIRQEPYTLPQGFTWDALDLGDRGVLKELYTLLNE bNMTS I sp/ret 40 FWDTQPVPKLGEVVNTHGPVEPDKDNIRQEPYTLPQGFTWDALDLGDRGVLKELYTLLNE mNMT I liver 120 FWDTQPVPKLGEVVNTHGPVEPDKDNIRQEPYTLPQGFTWDALDLGDRGVLKELYTLLNE hNMT I muscle 180 NYVEDDDNMFRFDYSPEFLLWALRPPGWLPQWHCGVRVVSSRKLVGFISAIPANIHIYDT hNMT I liver 180 NYVEDDDNMFRFDYSPEFLLWALRPPGWLPQWHCGVRVVSSRKLVGFISAIPANIHIYDT hNMTS I HepG2 98 NYVEDDDNMFRFDYSPEFLLWALRPPGWLPQWHCGVRVVSSRKLVGFISAIPANIHIYDT bNMT I retina 180 NYVEDDDNMFRFDYSPEFLLWALRPPGWLPQWHCGVRVVSSRKLVGFISAIPANIHIYDT bNMTS I sp/ret 98 NYVEDDDNMFRFDYSPEFLLWALRPPGWLPQWHCGVRVVSSRKLVGFISAIPANIHIYDT mNMT I liver 180 NYVEDDDNMFRFDYSPEFLLWALRPPGWLPQWHCGVRVVSSRKLVGFISAIPANIHIYDT hNMT I muscle 240 EKKMVEINFLCVHKKLRSKRVAPVLIREITRRVHLEGIFQAVYTAGVVLPKPVGTCRYWH hNMT I liver 240 EKKMVEINFLCVHKKLRSKRVAPVLIREITRRVHLEGIFQAVYTAGVVLPKPVGTCRYWH hNMTS I HepG2 157 EKKMVEINFLCVHKKLRSKRVAPVLIREITRRVHLEGIFQAVYTAGVVLPKPVGTCRYWH bNMT I retina 240 EKKMVEINFLCVHKKLRSKRVAPVLIREITRRVHLEGIFQAVYTAGVVLPKPVGTCRYWH bNMTS I sp/ret 157 EKKMVEINFLCVHKKLRSKRVAPVLIREITRRVHLEGIFQAVYTAGVVLPKPVGTCRYWH mNMT I liver 240 EKKMVEINFLCVHKKLRSKRVAPVLIREITRRVHLEGIFQAVYTAGVVLPKPVGTCRYWH hNMT I muscle 300 RSLNPRKLIEVKFSHLSRNMTMQRTMKLYRLPETPKTAGLRPMETKDIPVVHQLLTRYLK hNMT I liver 300 RSLNPRKLIEVKFSHLSRNMTMQRTMKLYRLPETPKTAGLRPMETKDIPVVHQLLTRYLK hNMTS I HepG2 216 RSLNPRKLIEVKFSHLSRNMTMQRTMKLYRLPETPKTAGLRPMETKDIPVVHQLLTRYLK bNMT I retina 300 RSLNPRKLIEVKFSHLSRNMTMQRTMKLYRLPETPKTAGLRPMEKKDIPVVHQLLSRYLK bNMTS I sp/ret 216 RSLNPRKLIEVKFSHLSRNMTMQRTMKLYRLPETPKTAGLRPMEKKDIPVVHQLLSRYLK mNMT I liver 300 RSLNPRKLIEVKFSHLSRNMTMQRTMKLYRLPETPKTAGLRPMEKKDIPVVHQLLSRYLK hNMT I muscle 360 QFHLTPVMSQEEVEHWFYPQENIIDTFVVENANGEVTDFLSFYTLPSTIMNHPTHKSLKA hNMT I liver 360 QFHLTPVMSQEEVEHWFYPQENIIDTFVVENANGEVTDFLSFYTLPSTIMNHPTHKSLKA hNMTS I HepG2 275 QFHLTPVMSQEEVEHWFYPQENIIDTFVVENANGEVTDFLSFYTLPSTIMNHPTHKSLKA bNMT I retina 360 QFHLTPVMSQEEVEHWFYPQENIIDTFVVENANGEVTDFLSFYTLPSTIMNHPTHKSLKA bNMTS I sp/ret 275 QFHLTPVMSQEEVEHWFYPQENIIDTFVVENANGEVTDFLSFYTLPSTIMNHPTHKSLKA mNMT I liver 360 QFHLTPVMNQEEVEHWFYPQENIIDTFVVENANGEVTDFLSFYTLPSTIMNHPTHKSLKA hNMT I muscle 420 AYSFYNVHTQTPLLDLMSDALVLAKMKGFDVFNALDLMENKTFLEKLKFGIGDGN-LQYY hNMT I liver 420 AYSFYNVHTQTPLLDLMSDALVLAKMKGFDVFNALDLMENKTFLEKLKFGIGDGN-LQYY hNMTS I HepG2 334 AYSFYNVHTQTPLLDLMSDALVLAKMKGFDVFNALDLMENKTFLEKLKFGIGDGN-LQYY bNMT I retina 420 AYSFYNVHTQTPLLDLMSDALVLAKMKGFDVFNALDLMENKTFLEKLKFGIGDGNNLQYY bNMTS I sp/ret 334 AYSFYNVHTQTPLLDLMSDALVLAKMKGFDVFNALDLMENKTFLEKLKFGIGDGN-LQYY mNMT I liver 420 AYSFYNVHTQTPLLDLMSDALVLAKMKGFDVFNALDLMENKTFLEKLKFGIGDGN-LQYY hNMT I muscle 479 LYNWKCPSMGAEKVGLVLQ* hNMT I liver 479 LYNWKCPSMGAEKVGLVLQ* bNMTS I HepG2 393 LYNWKCPSMGAEKVGLVLQ* bNMT I retina 480 LYNWKCPSMGAEKVGLVLQ* bNMTS I sp/ret 393 LYNWKCPSMGAEKVGLVLQ* mNMT I liver 479 LYNWKCPSMGAEKVGLVLQ* |