Table 1 of
Young, Mol Vis 2004;
10:163-176.Table 1 of
Young, Mol Vis 2004;
10:163-176.
Table 1. Primer pair sequence of select expressed scleral genes for RT-PCR
The 5' sense and 3' antisense polymerase chain reaction primer pairs designed for collagens 6A3 and 10A1, thrombospondins 2 and 4, dystroglycan, biglycan, and decorin with their corresponding amplicon sizes. In the first column, F indicates the forward primer and R indicates the reverse primer.
Primer Primer Product name Gene Sequence size size --------- ---------------- --------------------- ------ ------- COL6A3-F Collagen 6A3 AGGAGAAATTACTGCCGTACA 21 390 COL6A3-R Collagen 6A3 CGGCTGAACTCGTGAATAGGT 21 390 COL10A1-F Collagen 10A1 CCCAACACCAAGACACAGTTC 21 462 COL10A1-R Collagen 10A1 CCCTTTCTGTCCATTCATAC 20 462 THBS2-F Thrombospondin 2 AGGACGGGATTGGCGATG 18 453 THBS2-R Thrombospondin 2 GGTTGGCGTTGGAGATGTAGG 21 453 THBS4-F Thrombospondin 4 CCAGGTGGATTCCGTTCACAA 21 568 THBS4-R Thrombospondin 4 CGGGTAGCAGGGATGGTATT 20 568 DSGN-F Dystroglycan CTGCCTGTGCTGCGGATGAAC 21 374 DSGN-R Dystroglycan GGGCTTCTTATTGGCGATGTG 21 374 LUM-R Lumican CTCTTTCAGCCGATTGT 17 393 DCN-F Decorin AACCTTCACGCATTGAT 17 440 DCN-R Decorin AATCCCAACTTAGCCA 16 440 BGN-F Biglycan AACTACCTGCGCATCTC 17 436 BGN-R Biglycan TGACGCAGCGGAAAG 15 436 DCN-F Decorin AACCTTCACGCATTGAT 17 440 DCN-R Decorin AATCCCAACTTAGCCA 16 440 |