Figure 1. Confirmed scleral expression of genes tested by reverse transcription-polymerase chain reaction

The expressed genes tested were β-actin (control, A), collagens 6A3 (B) and 10A1 (C), thrombospondins 2 (D) and 4 (E), dystroglycan (F), biglycan (G), and decorin (H). The PCR amplicons were derived from cDNA from reverse-transcribed RNA of human ocular tissues and are commercially available as RNA from various human tissue types (column 1 is sclera, column 2 is cornea, column 3 is optic nerve, column 4 is retina, column 5 is lung, column 6 is skeletal muscle, column 7 is heart, column 8 is trachea, column 9 is kidney, and column 10 is brain).