Table 1 of
Mackay, Mol Vis 2004;
10:155-162.Table 1 of
Mackay, Mol Vis 2004;
10:155-162.
Table 1. PCR primers for mutation screening of CRYGA-CRYGD on 2q
Primer pairs used for amplification and sequencing of exons from the CRYGA-CRYGD gene cluster located on chromosome 2q.
Gene (Exon) Strand Sequence (5'-3')
----------- --------- ---------------------------
CRYGA (1-2) Sense TCCCTTTTGTGTTGTTTTTGCC
Antisense TATGGCCATGGATCATTGATGC
CRYGA (3) Sense TCGTTGACACCCAAGGATGCATGC
Antisense TACAAGAGCCACTTAGTGCAGGG
CRYGB (1-2) Sense TGCAAATCCCCTACTCACCAAAATGG
Antisense TAAAAGATGGAAGGCAAAGACAGAGCC
CRYGB (3) Sense TAGGGACTGGAGCTTTAATTTCC
Antisense TACTAGTGCCAGAAACACAAGC
CRYGC (1-2) Sense TGCAGGATGTTAAGAGATGC
Antisense TTCTCTGATGTCCATCTAAGC
CRYGC (3) Sense TATTCCATGCCACAACCTACC
Antisense TTGACAGAAGTCAGCAATTGC
CRYGD (1-2) Sense TCTTTTGTGCGGTTCTTGCCAACG
Antisense TACCATCCAGTGAGTGTCCTGAGG
Mutant TCAAGTAGGGCTGCAGGTTGGT
CRYGD (3) Sense TCTTTTTATTTCTGGGTCCGCC
Antisense TACAAGCAAATCAGTGCCAGG
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