Figure 5. The levels of mRNA for P21^WAF1 and P27^Kip1 were examined by RT-PCR

Lane M is the DNA molecular size marker. Lane 1 is sham treated cells for 4 h, lane 2 is sham treated cells for 6 h, lane 3 is sham treated cells for 8 h, lane 4 is cells treated with microwave for 4 h, lane 5 is cells treated with microwave for 6 h, and lane 6 is cells treated with microwave for 8 h. Expression of GAPDH mRNA was used as the internal standard. A: Levels of P27^Kip1 mRNA. B: Levels of P21^WAF1 mRNA. C: Quantitative analysis of mRNA by densitometric scanning (n=4). The error bars represent the standard deviation. Each displayed value was normalized against the density of the respective band of GAPDH. There were no significant differences in P27^Kip1 and P21^WAF1 mRNA levels between microwave treatment and control groups (p>0.05).