Figure 3 of
Ebong, Mol Vis 2004;
Figure 3. Growth factor-induced lens proliferation and gene transcription are mediated through activation of STAT1 signaling pathways
A: IGF-1 or PDGF-induced proliferation is repressed by inhibitors of JAK/STAT pathways. Quadruplet cultures of mouse lens epithelial cell explants were stimulated with IGF-1 (50 ng/ml) or PDGFaa (50 ng/ml) for 24 h in the presence or absence of 100 μM AG-490 (specific inhibitor of JAK/STAT pathways) or Genistein (100 μM) as indicated. Cells were pulsed with 3H-Thymidine after 6 h in culture and histograms represent the ratio of proliferative responses (CPM) between growth factor stimulated and control untreated cells. The bars indicate standard deviation of results from four explants. B: Western blot analysis of whole cell extracts (40 μg/ml) from mouse lens epithelial explants stimulated with IGF-1 in the presence or absence of AG-490. C: RT-PCR analysis of IRF-1 and ICSBP expression in 1AMLE6 lens epithelial cells stimulated with IGF-1, PDGFaa, or IFNγ. Samples were subjected to 25 cycles (β-Actin) or 35 cycles (IRF-1 and ICSBP) of amplification.