Table 1 of
Rao, Mol Vis 2004;
10:112-121.Table 1 of
Rao, Mol Vis 2004;
10:112-121.
Table 1. Primers used to amplify sequences
Expression of phox40, phox67, phox91, Rac1, and Rac2 in mouse and human lenses and expression of LMW-PTP in human lens or human lens epithelial cells was confirmed by RT-PCR using the sequence-specific forward and reverse oligonucleotide primer pairs shown below. Expression of p22 phox, p67phox, p91phox, and Rac1 in human lens was determined by polymerase chain reaction (PCR) using a cDNA library derived from adult human lens, using sequence specific forward and reverse oligonucleotide primer pairs.
Species Gene Primers (Forward/Reverse) Size
------- ---------- --------------------------- ------
mouse Rac1 CTGCCTGCTCATCAGTTACACG 485 bp
GGACAGAGAACCGCTCGGATA
Rac2 CAGGTCAGGAGGACTATGACCG 343 bp
GATTGCCTCATCGAAGACGGT
p40phox CAAAGTCTACATGGGCGCAAA 469 bp
TGTCTTCATAGAAGTAGCATCGTAGCC
p67phox CTATCTGGGCAAGGCTACGGTT 165 bp
CACAAAGCCAAACAATACGCG
gp91phox AGTCGGGATTTCTGACCGGTAT 423 bp
TCCAGTCTCCAACAATACGGATATG
human Rac1 CAAGAAGATTATGACAGATTACGCCC 217 bp
TCTTCTCCTTCAGTTTCTCGATCG
p22phox TTGTGTGCCTGCTGGAGTACC 128 bp
GCCCGAACATAGTAATTCCTGGTAA
p67phox AATGATAACTGGGCCACGGTC 279 bp
GCTGAGTCTTCATGACTACCGTGTACT
gp91phox ATCCATGGAGCTGAACGAATTG 428 bp
CTCTGTCCAGTCCCCAACGAT
LMW-PTPase TGTCTGGGTAACATTTGTCGATCA 405 bp
TGACACACTGCTGGTACACCG
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