Table 2 of
Davies, Mol Vis 2004;
10:1028-1037.Table 2 of
Davies, Mol Vis 2004;
10:1028-1037.
Table 2. Gene specific PCR primers
Primers were designed and tested as described in the Methods section.
Annealing
Tm temperature Product
Name Sequence (5'-3') (°C) (°C) size (bp)
------------- -------------------------- ---- ----------- ---------
Collagen V F: GGYTCCTGGTTCAGTGAA 58 53 420
R: CTCCTARCCYATGAAGCAAG 57
Collagen VI F: GGATACCAAGGCAACAACGGA 69 63 194
R: GCTGAGACCTCTCCAGCCA 67
Collagen VII F: GGTCTGCATCCTGATCACAGA 67 63 896
R: CCTCAGGCACCAAGTTCCA 68
Collagen VIII F: CCAGGAGTAGCAGGACTTCA 63 57 471
R: CTCGTCGTACGTGTACATCA 61
Connexin 43 F: TTCATCATCTTCATGCTGGT 62 58 368
R: TGGGAGTTAGAGATGGTGCT 62
Decorin F: CACCATCCCTCAAGGTCT 61 57 267
R: CAGGTTGTCTACCTTCATAACAA 61
Annexin II F: CCACCTCCAGAAAGT 51 45 306
R: GCAAGACACTAAGGG 49
Keratin 12 F: GCCTACATGAAGAAGAACCACGA 67 63 255
R: GACCTCGCTCTTGCTGGACT 67
NBC F: GTGCCAAGTGAGTTCAAGCCA 69 63 753
R: GACATCATCIAGGAAGCTGAG 61
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