Table 2 of
Sundaresan, Mol Vis 2004;
10:1005-1010.Table 2 of
Sundaresan, Mol Vis 2004;
10:1005-1010.
Table 2. Sequences of oligonucleotide primers used in the present study on TYR gene mutations in OCA1
The oligonucleotides used to assay for normal and variant allele of the TYR gene in each exon are illustrated. PCR amplification was performed in a 20 μl volume containing 1 U of Taq DNA polymerase, 200 μM dNTPs, 2 mM MgCl2, 50 pmol of primer. Thermocycling conditions were 94 °C for 13 min followed by 35 cycles of 94 °C for 1 min, 59 °C for 1 min, and 72 °C for 2 min, and finally 72 °C for 10 min.
Exon Forward primers (5'-3') Reverse primer (5'-3') ---- --------------------------- -------------------------- 1 CAAACTGAAATTCAATAACATATAAGG GTGGACAGCATTCCTTCTCC 1 TTCAGAGGATGAAAGCTTAAGATAAA CGTCTCTCTGTGCAGTTTGG 1 CTGGCCATTTCCCTAGAGC CCACCGCAACAAGAAGAGTC 1 CATCTTCGATTTGAGTGCCC CCCTGCCTGAAGAAGTGATT 2 CCAACATTTCTGCCTTCTCC TCAGCTAGGGTCATTGTCGAT 3 AGTTATAAATCAAATGGGATAATCA ACATTTGATAGGCACCCTCT 4 TAACCGGGAATCCTACATGG CAGGAATAACATTGTCGAAGCA 5 GGATGAAGATGATGGTGATCG AGATCTTTAAATAATCAGAAGGCAAA |