Table 4. Invasion relative fluorescence units in mouse melanoma cell lines with low concentration or high concentration angiostatin

There is a significant reduction in the in vitro invasion of Queens, B16F10, and B16LS9 melanoma cells cultured in low concentration (0.1 μg/μl) compared to high concentration (50 μg/μl) angiostatin. Asterisks mark a significant difference between the invasion relative fluorescence units for the angiostatin 0.1 μg/μl group and the angiostatin 50 μg/μl group (** for p<0.01; unpaired Student's t-test). Sharps mark a significant difference between the invasion RFU for one of the angiostatin groups and control group (## for p<0.01, # for p<0.05; unpaired Student's t-test).

                                Cell lines
              ---------------------------------------------
   Group         Queens          B16F10          B16LS9
-----------   -------------   -------------   -------------
PBS           2.23±0.06       1.81±0.003      2.04±0.02

Angiostatin   1.51±0.05**##   1.36±0.10**##   1.37±0.23**##
0.1 μg/μl

Angiostatin   2.58±0.23       3.33±0.83#      3.42±0.20##
50 μg/μl