Figure 3. Queens, B16F10, B16LS9, and metastatic B16LS9 melanoma cells produce VEGF

A: Western analysis shows that Queens, B16F10, B16LS9, and metastatic B16LS9 cell lines all produce VEGF (38 kDa). B: Western analysis shows that B16LS9 cells produce a higher level of VEGF when grown with high concentration (50 μg/μl) compared to low concentration (0.1 μg/μl) in vitro supplemental angiostatin. ME labels murine endothelium positive control.