Figure 8 of
Fischer, Mol Vis 2004;
Figure 8. Ciliary neurotrophic factor and insulin induce the expression of glial fibrillary acidic protein in Müller glia.
Ciliary neurotrophic factor (CNTF) and insulin induce the expression of glial fibrillary acidic protein (GFAP) in Müller glia. Retinas were obtained from eyes that received 3 consecutive daily injections of CNTF (A,D), saline (B), or insulin (C). Vertical sections of the peripheral retina were labeled with antibodies to neurofilament (NF-M; A), or GFAP (B-D). Sections were obtained from the peripheral retina >500 μm. The outer nuclear layer (ONL), inner nuclear layer (INL), inner plexiform layer (IPL), and nerve fiber layer (NFL) are labeled. The calibration bar in D represents 50 μm and applies to panels A-D. E: GFAP expression levels are increased in retinas from CNTF injected eyes compared to contralateral saline injected eyes. Fold difference was calculated as yx where y equals to 2 and x equals to C(T)NMDA treated minus C(T)control. The CT value is the PCR cycle number at which the reaction enters log phase and is inversely proportional to transcript abundance. CT values were averages of samples run in triplicate. GAPDH served as a control and showed no significant variation between samples.