Figure 7. β3 tubulin- and RA4-immunoreactivity can be induced in Müller glia by insulin and FGF2

Insulin and FGF2 induce β3 tubulin and RA4 immunoreactivity in presumptive Müller glia in peripheral regions of the retina. Vertical sections of retina were obtained from eyes that received with 3 consecutive daily injections of insulin alone (A,C) or insulin and FGF2 (B,D-H). Retinas were processed for immunocytochemistry 24 h after the final injection and labeled with antibodies to β3 tubulin (A,B), RA4 (C-F), or glutamine synthetase (G,H). Arrows in B indicate Müller glia that are immunoreactive for β3 tubulin and arrows in F-H indicate structures double labeled for RA4 and glutamine synthetase immunoreactivity. The outer nuclear layer (ONL), inner nuclear layer (INL), inner plexiform layer (IPL), ganglion cell layer (GCL), and nerve fiber layer (NFL) are labeled. The calibration bar in E represents 50 μm and applies to A, B, D, and E. The bar in H represents 50 μm and applies to C and F-H.