Figure 5 of
Khanobdee, Mol Vis 2004;
Figure 5. Subcellular fractionation of SH3BP4
NE-PER reagent was used to isolate nuclear fractions (NF) and post-nuclear fractions containing membranes and the cytosol (PNF) from ARPE-19 and COS-7 cells. A: Fractions and total cell lysates (TCL) were immunoblotted using the SH3BP4 antibody. A differential centrifugation protocol was used to isolate nuclear fractions (NF), membrane fractions (MF), and cytosol fractions (CF) from ARPE-19 cells. Fractions and total cell lysates (TCL) were immunoblotted using the SH3BP4 antibody. Qualitative analysis of these data was determined for the NE-PER fractionation (B,C) and the differential centrifugation fractionation (D) and shown relative to μg of total protein (B,D) or as total cellular levels of SH3BP4 (C). The fold level increases between the fractions are shown above each bar; levels that were not detectable (ND) are also noted.