Table 2 of
Paluru, Mol Vis 2004;
10:917-922.Table 2 of
Paluru, Mol Vis 2004;
10:917-922.
Table 2. LUM and FMOD gene primers designed for mutation screening
Lumican (LUM) and Fibromodulin (FMOD) gene polymerase chain reaction primer pairs designed for mutation screening. Six primer pairs were designed for LUM gene (3 Exons). Two primer pairs and three nested primers were designed for FMOD gene (2 Exons). The forward and reverse primers are identified by an "F" and "R" suffix, respectively.
Position
Exon Size on contig Primer Sequence 5'-3' Primer
-------- ---- --------- ------------------------------ -----------
Contig NT_019546
1 710 14987011 TTCTTTGCTCCAGTATTAGGGTCC LUM1F
14987721 AAAAATGTTCTCACAGTGAGCTTCC LUM1R
2A 919 14983585 TTAATTGTTCCTTATCTGGATAC LUM2AF
14984504 TGCAGCTTACTCATAACAAG LUM2AR
2B 787 14984369 GAAGCTCAAGTCAAGGTATTCG LUM2BF
14985156 CTAACCTGAAAGCTGCTCACTAC LUM2BR
2C 592 14984146 TTTTAAGCTTGTTATAGGACAGA LUM2CF
14984738 GCCTCCTGGAATCAAGTAT LUM2CR
3A 1262 14979221 TCACATAGCAGCTTCTTATCTA LUM3AF
14980483 TCTTGATCTACCATGATTACTCT LUM3AR
3B 547 14979222 CACATAGCAGCTTCTTATCTA LUM3BF
14979769 AAATACATATGGATGTTACTCTC LUM3BR
Contig NT_004671
1 1579 14671023 CATTTCCCAGCTGTCCCCCTACATCG FMOD1F
14672579 TCACCCCAAATAGCCATGGTCCG FMOD1R
2 2611 14664068 GAGTTGTAACATTTGCAACATGCTTGGTAC FMOD2F
14666650 CTAATCTCACTGTGGCTTCAGGTTCGTAA FMOD2R
2-Nested 14666058 CTGTGCTGGGCTGGTCGT FMOD2(589F)
2-Nested 14665613 AATGGGCCAAACACACCT FMOD(1034F)
2-Nested 14665126 TAACCCACCTGCCCTTTG FMOD(1521F)
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