Figure 4. Nuclear lamina disintegration during nuclear degradation

A-G: Monoclonal antibody L3f4 (anti-lamins). H-N: Hoechst 33342. O-U: Merge of lamins immunolabeling and Hoechst 33342 staining. A,H,O: Nucleus from a mid cortex fiber. B-D,I-K,P-R: Nuclear material from fibers at the inner cortex. E-G,L-N,S-U: Nuclear remnants from fibers located at the deep inner cortex. The onset of nuclear lamina disintegration varies with chromatin pattern but, in all cases, it occurs after the beginning of chromatin condensation and fragmentation processes. Scale bar represents 5 μm.