Table 2 of
Kumar, Mol Vis 2004;
10:910-916.Table 2 of
Kumar, Mol Vis 2004;
10:910-916.
Table 2. PCR primer sequences, annealing temperatures and amplicon sizes of 48 coding exons of the MYO7A gene
PCR products from all 48 coding exons of this gene were sequenced to identify the mutation in an affected individual. Once the mutation was identified, the rest of the family members were also examined for the presence of the mutation. * works with 10% DMSO. The forward (F) and reverse (R) primers are listed below.
Annealing
temperature Amplicon
Exon Primer sequence (5' to 3') (°C) size (bp)
------- ------------------------------ ----------- ---------
2 F: ccagccaggctcaaggcttcca 66 241
R: gcaggaattttccaagagaacacc
3 F: cagagggatatagggctgcctgga 66 296
R: catggcctccatctcctttcgatca
4* F: gtgtctggctgccagagaggtcga 58 426
R: agctgcacagcggacaaagtctcag
5 F: agcccaagagctttctagagtcaga 66 316
R: gcacagttggagctctaggtccta
6 & 7 F: ctgggctgagttccagttggtgg 63 512
R: ggagcaatacgggcagcaatacg
8 F: atcatcccaggctagttcctgatg 68 269
R: aaggctgagtctgcagtagccag
9 F: gggtacactgacgtcctcttgcac 68 260
R: ggcactgcactgcccttggcgca
10 F: gtggcagcctagtcctcttaggac 68 214
R: aacccttcagagggacagaagtcatg
11 F: ggggcaggctggcaggtgagcac 68 212
R: acttcccaaggggtagggcgagcaa
12* F: caagggctggagcgacaccacg 58 285
R: tccatattggggaaggaaattcccatg
13 F: ggtggggcctgaacaacacccttac 68 318
R: aagcagggaaggaagctgtgcgcac
14 F: catggaggagagggtgggctcaca 68 239
R: gagcaggggaaggcagggccacg
15 F: gagggcctgccagagctggtgaga 68 219
R: gcttagactcaggcctggcccgtg
16 F: ggcaggcacagcccctcccatcg 68 225
R: gtcaccctagccgccacccgcca
17 F: cccagcaggagccttggccctga 58 252
R: aagctgggaccctcccctcctgc
18 F: cccactggagaggctgtccattcc 68 207
R: cccagcccacatcatgggaatttaca
19 F: ccactgggactgagcaggtggtc 68 218
R: acacgtacacctgtatgtgggctga
20 F: atcccaaacccacctgtaccctgg 68 202
R: ctgggttcaaaggcctgtttgggca
21 F: ggaatgggacagcaggctctgagc 68 318
R: gacactcctcgcccaggggtcaga
22 F: ggtgggaatccctgcaacaacagc 68 223
R: ggacaagccagcaagtggacaccta
23 F: agcttgttccctgaggctgtggca 68 323
R: agcggtgtgtgtgggcctctgga
24 F: gcttcctgagtagctgggactcca 68 332
R: tacggccaccaccagcagcagag
25 F: agcaatgtcctccgctctggcctc 68 270
R: cttgtcggcctgggagggacatca
26 F: ttgctttctgctcagccacttgacc 67 206
R: ctgtgctccagcctaggccaccta
27 F: gggaacacccctaactttacctgc 67 236
R: aggcaccactcagccaccaaaaga
28 F: gggctgttcctgtggggtgattcc 67 223
R: ggcctagctccccttcccactcca
29 F: gagggcctggcggctgccctca 67 286
R: ctggggcactcgagtgcactgga
30 F: gtctgaagggaagggaccccacaa 67 300
R: caagtaggtggcagcactggcagc
31 F: ccttccctgactctgtgcctgctc 67 325
R: agcaggaggctgtgagccaagcca
32 F: ttggtggtgtggaagggcttcctg 67 285
R: gttcaggtccacatcccttctctag
33* F: caaactcactcgtatgttgtcttctg 58 218
R: gctggagctacagagcaagggac
34 F: cctgggtgtgggaggcctgcctc 67 222
R: ccctctccttcccctctgtctgtc
35 F: cccactggttggggcatgactgac 63 377
R: accctgacccccagtgccaggca
36 F: ctcagcctgtctctgcccccatgg 68 330
R: ctagaccgttaacctattgtacagctg
37 F: ccacaggtagagagctgacctgag 68 238
R: accagacagtagcggaagcctgca
38 F: tgccagcgatggggcgttgctga 68 276
R: ggaggaaggcagtgtgcagacgaa
39 F: agtgctccctctattcggcacaag 68 252
R: cccgtcacagcaggtgaggtctg
40 F: tcctgtgactcccgatggcagctg 68 294
R: atccccaaggggctcatcccacaa
41 F: gtctccacagtcccacgcacatgc 68 201
R: gccctgtcctgggcactgcagac
42 F: gctcagtataggaggcatagccaga 66 268
R: tgtccgtaagttcctatggccccag
43 & 44 F: agctgccagacaggccttaggtgg 66 505
R: agcaacgctagctgtgcacgaagg
45 F: tctgccaggtccctgcacgcctgt 68 326
R: cctgggctgctggccagtgtctg
46* F: ctggcctgccctgagcaggcctg 58 228
R: atgccctgttcccctcctcctctg
47 F: cttgctctgggcccccatctgatg 66 235
R: ctagagatagatggtggctaggagg
48 F: agggcccaggccgtgcctctcta 66 216
R: tacgctgcaggcaaggcagggagc
49 F: tggccctgtcccaccgtgtgctc 68 193
R: gaacccactgctgcctcgagagc
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