Figure 2. Cellular expression patterns of human KLFs in cornea and conjunctiva

The expression pattern of the different KLFs was determined by RT-PCR on total mRNA extracted from human corneal epithelial cell line (HCE); keratinocytes; human cornealendothelial cell line (HTEC); human epithelial conjunctival cell line (CHANG). Positive control (GADPH) was performed under the same conditions but using oligonucletide primers specific to the housekeeping gene GADPH. Negative control was performed in the absence of oligonucleotides or matrix.