Figure 3 of
Peterson, Mol Vis 2004;
10:857-866.
Figure 3. Digestion of crosslinked species was judged complete using HPLC
Progress of trypsin digestion of DTSSP cross-linked and modified α-crystallin monitored by HPLC at 215 nm on Vydac reverse phase C18. A: Sample before digestion. B: Trypsin digestion at 37 °C for 45 min. C: Trypsin digestion at 37 °C for 109 min. D: Trypsin digestion at 37 °C for 23 h.