Figure 1. Native α-crystallin cross linking and modification monitored by SDS-PAGE

Protein reaction samples (8.1 μg) from each cross linking reaction using DTSSP, formaldehyde (FA), or no cross linker (NXLR) were subjected to reducing (A) and non-reducing (B) SDS-PAGE. Limited cross-linking was observed. Cross-links involving DTSSP were reducible, as expected. Molecular weight standards (left) were also resolved.