Figure 6. Western blot analysis of L-DNase II expression in ARPE-19 cells

The total protein extracts were separated on 13% SDS-PAGE gel, transferred, and stained by enhanced chemiluminescence method with a polyclonal antibody directed against 181-191 peptide of LEI. The extracts were isolated from cells that had been incubated without (lane 1) or with 10% ethanol (lane 2) for 24 h. Each lane of the gel was loaded with 40 microgram of protein. The molecular weight is indicated on the left.