The N54 mutant of Galphas has a conditional dominant negative phenotype which suppresses hormone-stimulated but not basal cAMP levels

FEBS Lett. 1999 Jan 25;443(2):205-8. doi: 10.1016/s0014-5793(98)01704-9.

Abstract

The phenotype of a Ser to Asn mutation at position 54 of the alpha subunit of G(s)(N54-alpha(s)) was characterized in transient transfection experiments in COS and HEK293 cells. Expression of either wild type or N54-alpha(s) increased basal cAMP levels. In contrast, expression of wild type alpha(s), potentiated agonist-stimulated cAMP levels, while expression of N54-alpha(s)caused a decrease. Thus, the N54-alpha(s) mutant possesses a conditional dominant negative phenotype, suppressing preferentially hormone-stimulated effects.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • COS Cells
  • Cattle
  • Cell Line
  • Cyclic AMP / metabolism*
  • GTP-Binding Proteins / genetics
  • GTP-Binding Proteins / metabolism*
  • Humans
  • Mutation*
  • Phenotype
  • Rats
  • Receptors, Thyrotropin / metabolism
  • Thyrotropin / metabolism*

Substances

  • Receptors, Thyrotropin
  • Thyrotropin
  • Cyclic AMP
  • GTP-Binding Proteins