Abstract
Water-soluble and urea-soluble protein fractions from control and streptozotocin-diabetic rats were analyzed for AGEs with a CML-specific monoclonal anti-AGE antibody and a polyclonal anti-AGE antibody. AGEs, CML in particular, were significantly increased in the diabetic rats whereas aminoguandine treatment resulted in significant decrease in AGEs. The data also confirm that CML, a glycoxidation product, is a major epitope of AGE structures in lenses.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibody Specificity
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Diabetes Mellitus, Experimental / metabolism*
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Enzyme-Linked Immunosorbent Assay
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Glycation End Products, Advanced / antagonists & inhibitors*
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Glycation End Products, Advanced / immunology
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Guanidines / pharmacology*
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Lens, Crystalline / metabolism*
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Rats
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Rats, Sprague-Dawley
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Streptozocin
Substances
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Glycation End Products, Advanced
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Guanidines
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Streptozocin
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pimagedine