Abstract
A group of cDNAs isolated from a subtractive ciliary body library of a normal human eye donor revealed 100% identity with TIGR a candidate gene responsible for juvenile open zangle glaucoma [Science 275 (1997) 668-670]. Several structural features of the deduced human protein have been noted: a cleavable N-terminal signal peptide, a periodic repetition at the N-terminus of leucine and arginine residues at every seventh and eleven position respectively in helix conformation (leucine zipper-like motif) exhibiting homology with myosin, and with olfactomedin in the C-terminus. The mRNA for TIGR is abundantly expressed in the ciliary body, iris, heart and skeletal muscle.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Ciliary Body / chemistry
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Ciliary Body / physiology*
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Cloning, Molecular
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Cytoskeletal Proteins
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DNA, Complementary / genetics*
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Extracellular Matrix Proteins / genetics
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Eye / chemistry
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Eye Proteins / genetics*
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Gene Library
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Glaucoma, Open-Angle / genetics*
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Glycoproteins / genetics
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Humans
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Molecular Sequence Data
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Myosins / genetics
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Organ Specificity
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RNA, Messenger / analysis
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Sequence Homology, Amino Acid*
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Sequence Homology, Nucleic Acid
Substances
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Cytoskeletal Proteins
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DNA, Complementary
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Extracellular Matrix Proteins
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Eye Proteins
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Glycoproteins
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RNA, Messenger
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olfactomedin
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trabecular meshwork-induced glucocorticoid response protein
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Myosins