Abstract
To characterize the relaxation of pericytes induced by beta-adrenergic stimulation, changes in the contractile tone of pericytes were quantified as a change in the wrinkling of an elastic silicone surface on which they were cultured. Isoproterenol produced relaxation of pericytes in a dose-dependent manner over a range of 5 nM to 1 microM. Low concentrations of the nonselective beta-blockers propanolol and timolol blocked the relaxation produced by isoproterenol. The specific beta 2-adrenergic component of isoproterenol-induced relaxation was shown by blockage with bromoacetyl alprenolol menthane. In contrast, atenolol and betaxolol, as relatively selective beta 1-adrenergic blockers, had no effect on the isoproterenol-induced relaxation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adrenergic beta-Agonists / pharmacology
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Adrenergic beta-Antagonists / pharmacology
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Animals
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Blood Flow Velocity / drug effects
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Capillaries / cytology
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Capillaries / metabolism*
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Cattle
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Cells, Cultured
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Dose-Response Relationship, Drug
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Glaucoma / metabolism*
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Isoproterenol / pharmacology
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Muscle Contraction / drug effects
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Muscle, Smooth, Vascular / cytology
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Muscle, Smooth, Vascular / metabolism
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Optic Nerve / blood supply
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Receptors, Adrenergic, beta / drug effects
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Receptors, Adrenergic, beta / metabolism*
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Retinal Vessels / cytology
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Retinal Vessels / metabolism*
Substances
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Adrenergic beta-Agonists
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Adrenergic beta-Antagonists
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Receptors, Adrenergic, beta
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Isoproterenol