The identification of a novel cDNA preferentially expressed in the olfactory-limbic system of the adult rat

Brain Res. 1996 May 20;721(1-2):217-28. doi: 10.1016/0006-8993(96)00176-x.

Abstract

To analyze cell-specific brain gene expression, we have developed a PCR-based subtractive hybridization cloning method utilizing trace starting material, allowing isolation of novel genes expressed under specific conditions. Our previous studies indicated that local substantia nigra (SN) type 1 astrocytes elaborate an array of trophic molecules which support the survival of SN dopaminergic neurons. Therefore, the current study focused on astrocyte gene expression utilizing a type 1 astrocyte-enriched cDNA library. We report initial characterization of a novel cDNA, designated AT1-46, that is preferentially expressed in the olfactory-limbic system of the adult rat brain. Although AT1-46 is expressed widely in the periphery, it is regulated both developmentally and in a cell-specific fashion in the brain. Structurally, AT1-46 is predicted to encode a highly alpha-helical molecule with several domains of potential coiled coil formation, and exhibits a 28% amino acid sequence identity with the intermediate filament-associated protein, trichohyalin.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Astrocytes / metabolism
  • Base Sequence
  • Blotting, Northern
  • Cells, Cultured
  • Cloning, Molecular
  • DNA, Complementary / biosynthesis*
  • Gene Library
  • In Situ Hybridization
  • Limbic System / metabolism*
  • Molecular Sequence Data
  • Olfactory Bulb / metabolism*
  • Polymerase Chain Reaction
  • Protein Structure, Secondary
  • RNA / biosynthesis
  • RNA / isolation & purification
  • Rats
  • Sequence Homology, Amino Acid

Substances

  • DNA, Complementary
  • RNA

Associated data

  • GENBANK/U33472