Abstract
The adenomatous polyposis coli gene (APC) is mutated in familial adenomatous polyposis and in sporadic colorectal tumors, and its product binds to the adherens junction protein beta-catenin. Overexpression of APC blocks cell cycle progression. The APC-beta-catenin complex was shown to bind to DLG, the human homolog of the Drosophila discs large tumor suppressor protein. This interaction required the carboxyl-terminal region of APC and the DLG homology repeat region of DLG. APC colocalized with DLG at the lateral cytoplasm in rat colon epithelial cells and at the synapse in cultured hippocampal neurons. These results suggest that the APC-DLG complex may participate in regulation of both cell cycle progression and neuronal function.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenomatous Polyposis Coli Protein
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Amino Acid Sequence
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Animals
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Cell Cycle
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Cells, Cultured
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Colon / chemistry
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Colon / cytology
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Cytoskeletal Proteins / analysis
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Cytoskeletal Proteins / chemistry
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Cytoskeletal Proteins / metabolism*
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Drosophila
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Drosophila Proteins*
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Epithelial Cells
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Epithelium / chemistry
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Fluorescent Antibody Technique
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Hippocampus / chemistry
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Hippocampus / cytology
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Humans
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Insect Hormones / analysis
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Insect Hormones / chemistry
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Insect Hormones / metabolism*
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Mice
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Molecular Sequence Data
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Neurons / chemistry
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Neurons / cytology
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Protein Binding
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Recombinant Fusion Proteins / metabolism
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Synapses / chemistry
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Trans-Activators*
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Tumor Suppressor Proteins*
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beta Catenin
Substances
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Adenomatous Polyposis Coli Protein
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CTNNB1 protein, human
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CTNNB1 protein, mouse
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Cytoskeletal Proteins
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Drosophila Proteins
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Insect Hormones
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Recombinant Fusion Proteins
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Trans-Activators
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Tumor Suppressor Proteins
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beta Catenin
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dlg1 protein, Drosophila