Abstract
In the vertebrate photoreceptor cell, rod outer segment (ROS) is the site of visual signal-transduction process, and a pivotal molecule that regulates this process is cyclic GMP. Cyclic GMP controls the cationic conductance into the ROS, and light causes a decrease in the conductance by activating hydrolysis of the cyclic nucleotide. The identity of the granylate cyclase (ROS-GC) that synthesizes this pool of cyclic GMP is unknown. We now report the cloning, expression and functional characterization of a DNA from bovine retina that encodes ROS-GC.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Adenosine Triphosphate / pharmacology
-
Amino Acid Sequence
-
Animals
-
Atrial Natriuretic Factor / pharmacology
-
Base Sequence
-
Blotting, Northern
-
Cattle
-
Cloning, Molecular
-
DNA, Complementary / chemistry
-
DNA, Complementary / genetics
-
Guanylate Cyclase / chemistry
-
Guanylate Cyclase / genetics
-
Guanylate Cyclase / metabolism*
-
Humans
-
Hydrolysis
-
Membrane Proteins / chemistry
-
Membrane Proteins / genetics
-
Membrane Proteins / metabolism
-
Molecular Sequence Data
-
Polymerase Chain Reaction
-
Retina / enzymology
-
Rod Cell Outer Segment / enzymology*
-
Sequence Alignment
Substances
-
DNA, Complementary
-
Membrane Proteins
-
Atrial Natriuretic Factor
-
Adenosine Triphosphate
-
Guanylate Cyclase