Two membrane forms of guanylyl cyclase found in the eye

Proc Natl Acad Sci U S A. 1995 Jan 17;92(2):602-6. doi: 10.1073/pnas.92.2.602.

Abstract

The cDNAs for two membrane guanylyl cyclases, designated E (GC-E) and F (GC-F, were isolated from a rat eye cDNA library. Their deduced topographic structures correspond to known members of the guanylyl cyclase receptor family, containing an extracellular domain, a single membrane-spanning domain, a protein kinase-like domain, and a cyclase catalytic domain. GC-E was expressed in the eye and the pineal gland, whereas GC-F expression was confined to the eye. Overproduction of GC-E and GC-F in COS cells resulted in expression of guanylyl cyclase activity, but ligands known to activate other guanylyl cyclase receptors failed to stimulate enzyme activity. Thus, both GC-E and GC-F remain orphan receptors. Amino acid sequence similarity between GC-E and GC-F in the extracellular region and homology with a cyclase expressed in olfactory neurons and retGC, a rod outer-segment-specific cyclase, suggest that there is another subfamily of guanylyl cyclase receptors, possibly restricted to sensory tissues.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Eye / enzymology*
  • Gene Library
  • Guanylate Cyclase / biosynthesis
  • Guanylate Cyclase / genetics*
  • Isoenzymes / genetics*
  • Membrane Proteins / biosynthesis
  • Membrane Proteins / genetics*
  • Membranes / enzymology
  • Molecular Sequence Data
  • RNA, Messenger / genetics
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Cell Surface*
  • Receptors, Enterotoxin
  • Receptors, Guanylate Cyclase-Coupled
  • Receptors, Peptide / genetics
  • Recombinant Proteins / biosynthesis
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • DNA, Complementary
  • Isoenzymes
  • Membrane Proteins
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, Peptide
  • Recombinant Proteins
  • Guanylate Cyclase
  • Gucy2e protein, rat
  • Gucy2f protein, rat
  • Receptors, Enterotoxin
  • Receptors, Guanylate Cyclase-Coupled

Associated data

  • GENBANK/L36029
  • GENBANK/L36030