Abstract
High endothelial venules (HEV) in lymphoid tissues support high levels of lymphocyte extravasion from the blood. We purified high endothelial cells from human tonsils by immunomagnetic selection with MECA-79 MAb to construct an HEV cDNA library. Differential screening of this library using cDNA probes from HEV (plus) or flat-walled vessel (minus) endothelial cells allowed us to characterize a novel human cDNA expressed to high levels in HEV. The cDNA encodes a secreted acidic calcium-binding glycoprotein of 664 aa residues, designated hevin, exhibiting 62% identity with the antiadhesive extracellular matrix protein SPARC, over a region of 232 aa spanning more than four fifths of the SPARC coding sequence. The primary structure and sequence of hevin and similar to SPARC-like proteins from rat and quail, called SC1 or QR1. Hevin could contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Activated-Leukocyte Cell Adhesion Molecule
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal / immunology
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Base Sequence
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Calcium-Binding Proteins / chemistry
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Calcium-Binding Proteins / genetics*
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Calcium-Binding Proteins / immunology
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Cell Adhesion
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Cloning, Molecular
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Coturnix
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DNA, Complementary / genetics
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Endothelium, Vascular / cytology
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Endothelium, Vascular / metabolism*
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Extracellular Matrix Proteins / chemistry
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Eye Proteins / chemistry
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Fluorescent Antibody Technique
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Gene Library
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Genes
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Glycoproteins / chemistry
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Glycoproteins / genetics*
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Glycoproteins / immunology
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Humans
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Immunomagnetic Separation
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In Situ Hybridization
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Molecular Sequence Data
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Multigene Family*
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Nerve Tissue Proteins / chemistry
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Osteonectin / chemistry*
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Palatine Tonsil / cytology
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RNA, Messenger / genetics
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Rats
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Sequence Alignment
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Sequence Homology, Amino Acid
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Umbilical Veins
Substances
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Activated-Leukocyte Cell Adhesion Molecule
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Antibodies, Monoclonal
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Calcium-Binding Proteins
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DNA, Complementary
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Extracellular Matrix Proteins
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Eye Proteins
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Glycoproteins
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Nerve Tissue Proteins
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Osteonectin
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QR1 protein, Coturnix japonica
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RNA, Messenger
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SPARCL1 protein, human
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Sparcl1 protein, rat