Cloning from purified high endothelial venule cells of hevin, a close relative of the antiadhesive extracellular matrix protein SPARC

Immunity. 1995 Jan;2(1):113-23. doi: 10.1016/1074-7613(95)90083-7.

Abstract

High endothelial venules (HEV) in lymphoid tissues support high levels of lymphocyte extravasion from the blood. We purified high endothelial cells from human tonsils by immunomagnetic selection with MECA-79 MAb to construct an HEV cDNA library. Differential screening of this library using cDNA probes from HEV (plus) or flat-walled vessel (minus) endothelial cells allowed us to characterize a novel human cDNA expressed to high levels in HEV. The cDNA encodes a secreted acidic calcium-binding glycoprotein of 664 aa residues, designated hevin, exhibiting 62% identity with the antiadhesive extracellular matrix protein SPARC, over a region of 232 aa spanning more than four fifths of the SPARC coding sequence. The primary structure and sequence of hevin and similar to SPARC-like proteins from rat and quail, called SC1 or QR1. Hevin could contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Activated-Leukocyte Cell Adhesion Molecule
  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / immunology
  • Base Sequence
  • Calcium-Binding Proteins / chemistry
  • Calcium-Binding Proteins / genetics*
  • Calcium-Binding Proteins / immunology
  • Cell Adhesion
  • Cloning, Molecular
  • Coturnix
  • DNA, Complementary / genetics
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / metabolism*
  • Extracellular Matrix Proteins / chemistry
  • Eye Proteins / chemistry
  • Fluorescent Antibody Technique
  • Gene Library
  • Genes
  • Glycoproteins / chemistry
  • Glycoproteins / genetics*
  • Glycoproteins / immunology
  • Humans
  • Immunomagnetic Separation
  • In Situ Hybridization
  • Molecular Sequence Data
  • Multigene Family*
  • Nerve Tissue Proteins / chemistry
  • Osteonectin / chemistry*
  • Palatine Tonsil / cytology
  • RNA, Messenger / genetics
  • Rats
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Umbilical Veins

Substances

  • Activated-Leukocyte Cell Adhesion Molecule
  • Antibodies, Monoclonal
  • Calcium-Binding Proteins
  • DNA, Complementary
  • Extracellular Matrix Proteins
  • Eye Proteins
  • Glycoproteins
  • Nerve Tissue Proteins
  • Osteonectin
  • QR1 protein, Coturnix japonica
  • RNA, Messenger
  • SPARCL1 protein, human
  • Sparcl1 protein, rat

Associated data

  • GENBANK/X82157