Abstract
Angiogenesis depends on the adhesive interactions of vascular cells. The adhesion receptor integrin alpha v beta 3 was identified as a marker of angiogenic vascular tissue. Integrin alpha v beta 3 was expressed on blood vessels in human wound granulation tissue but not in normal skin, and it showed a fourfold increase in expression during angiogenesis on the chick chorioallantoic membrane. In the latter assay, a monoclonal antibody to alpha v beta 3 blocked angiogenesis induced by basic fibroblast growth factor, tumor necrosis factor-alpha, and human melanoma fragments but had no effect on preexisting vessels. These findings suggest that alpha v beta 3 may be a useful therapeutic target for diseases characterized by neovascularization.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal
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Blood Vessels / metabolism
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Chick Embryo
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Fibroblast Growth Factor 2 / pharmacology
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Granulation Tissue / blood supply*
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Granulation Tissue / metabolism
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Humans
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Integrins / biosynthesis
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Integrins / immunology
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Integrins / physiology*
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Laminin / analysis
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Melanoma / blood supply
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Melanoma / metabolism
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Neovascularization, Pathologic / metabolism*
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Receptors, Cytoadhesin / biosynthesis
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Receptors, Cytoadhesin / immunology
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Receptors, Cytoadhesin / physiology*
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Receptors, Vitronectin
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Skin / blood supply
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Skin / metabolism
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Tumor Necrosis Factor-alpha / pharmacology
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von Willebrand Factor / analysis
Substances
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Antibodies, Monoclonal
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Integrins
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Laminin
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Receptors, Cytoadhesin
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Receptors, Vitronectin
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Tumor Necrosis Factor-alpha
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von Willebrand Factor
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Fibroblast Growth Factor 2