Abstract
The B7-1 molecule, expressed on antigen presenting cells (APC), provides a crucial costimulatory signal for T cell activation. Recent studies demonstrate the existence of alternative, non-B7-1 CTLA4 counter-receptors in mice and humans. Here, we describe the molecular cloning and demonstrate costimulatory function of the murine B7-2 (mB7-2) gene. Murine B7-2 cDNA encodes a member of the Ig supergene family that binds CTLA4-Ig and stains with the GL1 but not anti-mB7-1 mAb. Murine B7-2 costimulates the proliferation and interleukin 2 production of CD4+ T cells and this costimulation can be inhibited by either CTLA4-Ig or GL1 mAb. Identification of the B7-2 molecule will permit further manipulation of the B7:CD28/CTLA4 costimulatory pathway which has been shown to be involved in the prevention of tolerance, induction of tumor immunity, and most recently, in the pathogenesis of autoimmunity.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Abatacept
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal
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Antigens, CD*
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Antigens, Differentiation / metabolism*
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B-Lymphocytes / chemistry
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B-Lymphocytes / immunology*
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B7-1 Antigen / genetics*
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B7-1 Antigen / metabolism
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B7-2 Antigen
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CTLA-4 Antigen
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Cloning, Molecular
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DNA, Complementary / genetics
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Immunoconjugates*
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Interleukin-2 / biosynthesis*
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Lymphocyte Activation*
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Membrane Glycoproteins*
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Mice
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Molecular Sequence Data
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Receptors, Cell Surface / metabolism*
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Sequence Alignment
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Sequence Homology, Amino Acid
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T-Lymphocytes / immunology*
Substances
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Antibodies, Monoclonal
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Antigens, CD
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Antigens, Differentiation
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B7-1 Antigen
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B7-2 Antigen
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CTLA-4 Antigen
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CTLA4 protein, human
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Cd86 protein, mouse
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Ctla4 protein, mouse
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DNA, Complementary
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Immunoconjugates
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Interleukin-2
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Membrane Glycoproteins
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Receptors, Cell Surface
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Abatacept